Assessment of chronic diabetic foot ulcers microbiome

• Elena Yu. Komelyagina
• Mikhail B. Antsiferov
• Popova V.M.
• Zhilenkov E.L.
• Yuskevich V.V.

Abstract

Aim - to compare the microbiome of chronic diabetic foot ulcers (DFU) without signs of infection using conventional culture techniques and mass-spectrometry approach of microbial markers.

Material and methods. The samples from 17 DFU without signs of infection were sent to Microbiology labo­ratory in order to obtain the presence of microorganisms using CCT and to chemical laboratory for performing MSMM simultaneously.

Results. The CCT revealed presence of gram-positive cocci in 8 cases (45%) including MRSA, gram-negative bacilli in 2 samples (11%), polymicrobial infection in 6 cases (33%). Anaerobes were not obtained. Obtained number of microorganisms did not exceed the critical colonisation. Mixed genera were identified in 13 samples using MSMM approach. The most commonly detected bacteria were gram-positive cocci, gram-negative bacilli, facultative anaerobes.

Conclusion. Mass-spectrometry of microbial markers technique revealed new information about diversity of bacteria in chronic not infected neuropathic diabetic foot ulcers and could be added to the diagnostic tools for non healing diabetic foot ulcers.

Keywords:diabetic foot, non healing ulcers, microbiome, conventional culture technique, mass-spectrometry

All articles in our journal are distributed under the Creative Commons Attribution 4.0 International License (CC BY 4.0 license)